Fig. 2.

Carbachol does not modulate ISO-induced increases in cAMP. The histogram shows average ± SEM cAMP levels (in picomoles per milligram of protein) from five separate experiments in which hippocampal slices (from the same animal) either were untreated (open bar) or were exposed to a 10 min bath application of 200 nm ISO alone, 200 nm carbachol (CCh) alone, or ISO plus carbachol (filled bars). Compared with basal levels of cAMP seen in untreated control slices, only ISO alone and ISO plus carbachol induced significant increases in cAMP levels (*p < 0.05). In four of these experiments a pair of slices also was exposed to a 10 min bath application of 50 μm forskolin. In these slices cAMP levels were increased to 92.5 ± 13.1 pmol/mg of protein.