Fig. 2.

Identification of wild-type and FAD-mutant hAPP mice with matching levels of cerebral transgene expression.A, Representative autoradiograph showing results of an RNase protection assay. Total RNA was extracted from entire hemibrains. The left lane shows signals of undigested radiolabeled riboprobes; the other lanes contained the same riboprobes plus brain RNA samples, digested with RNases. Each samplelane contains RNA from a different mouse. The hAPP probe detects human but not mouse APP; it also recognizes an SV40 segment (S) of transgene-derived mRNAs.Non-tg, Nontransgenic. B, Phosphorimager quantitation of signals shown in A. Values represent group means ± SD.