Fig. 7.
Expression of mKv4.2dn suppressed the A-type current of cerebellar granule cells.A1, Cerebellar granule cells transfected with EGFP in the microexplant culture exhibited a large transient and maintained outward current by a series of depolarizing pulses of −60 to +40 mV. A2, The transient component of the current can be inactivated with a prepulse to −20 mV. A3, Isolated A-type current was obtained by subtracting A2 fromA1.B1–B3, Cotransfection of mKv4.2dn and EGFP results in a marked suppression of the transient component without affecting the maintained component of outward currents. C, Quantitative analysis indicated the suppression of A-type current and no effect on delayed rectifier current in the peak density evoked at 20 mV. Mean ± SEM is displayed. ***p < 0.001 versus control cells.D, Voltage–current density relationship for A-type currents recorded from control cells (filled circles) and mKv4.2dn-transfected cells (open circles). Mean ± SEM is displayed.