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cAMP-induced release of NT-3 from AtT-20 cells expressing α1-PDX. Cells were infected with VV:pro-NT-3 for 1 hr, incubated in virus-free medium for 8 hr, metabolically labeled for 3 hr, chased for 3 hr, and treated for 3 hr with medium with or without 5 mm cAMP. CL and CM were immunoprecipitated and the amount of processed, mature NT-3 was analyzed by SDS-PAGE. Analysis was performed on a PhosphorImager, and values represent an average (±SEM) of three independent experiments.
