Fig. 6.

Overexpression of NT-3 results in rerouting from the constitutive to the regulated secretory pathway. AtT-20 cells were transfected with a construct encoding either pro-NT-3 (A, B) or pro-BDNF (C), and immunocytochemistry using NT-3 (A, B) and BDNF (C) antibodies was performed as described in Figure 5. In A, cells were lipofectamine-transfected with 0.1 μg of pro-NT-3 DNA (and 1.9 μg vector DNA). In B, cells were transfected with 2 μg pro-NT-3 DNA. In C, cells were transfected with 0.1 μg of pro-BDNF (and 1.9 μg vector DNA). Scale bar, 10 μm.