Fig. 8.

NT-3/BDNF is retained in hippocampal neurons.A, The methodology in Figure 1A(involving a 4 hr chase only) was repeated three times with cells infected with either VV:pro-NT-3/VV:WT or VV:pro-NT-3/VV:pro-BDNF. The NGF antibody was used for immunoprecipitations. Results were analyzed on a PhosphorImager and are an average (±SEM) of the ratio of mature NT-3 in cell lysates (CL) over the total amount of NT-3 in CL + conditioned medium (CM).B, A representative SDS gel from the experiments inA showing the NT-3/BDNF heterodimer in the cell lysate and conditioned medium.