Skip to main content
. 2000 May 15;20(10):3580–3587. doi: 10.1523/JNEUROSCI.20-10-03580.2000

Fig. 4.

Fig. 4.

PSD-95 mutants defective in intramolecular SH3–GK interaction show normal multimerization and membrane association.A, COS cell lysates doubly transfected with N-PSD-95-GFP (containing the N-terminal multimerization domain of PSD-95) and wild-type or mutant PSD-95, as indicated, were immunoprecipitated with PSD-95 (guinea pig) antibody and probed with PSD-95 (top panel) and myc antibodies (for N-PSD-95-GFP,bottom panel). B, COS cells were transfected with wild-type or mutant (L460P or ΔC) PSD-95. Membrane (Memb) and cytosolic S100 fractions (S100) were prepared as described in Materials and Methods. The membranes were further extracted with isotonic homogenate buffer (HB), 1 m sodium chloride (NaCl), or alkaline sodium carbonate buffer (pH 11) to yield pellet (P) and soluble fractions (S).