Fig. 6.
PACAP promotes the expression of glial glutamate transporters. Third passage cortical glia were maintained for 3 d in the absence or presence of PACAP (10−7m) or dbcAMP (10−4m) and immunoblotted with anti-GLT-1 antibodies (1:4000; A) or anti-GLAST antibodies (1:1000; B). In each lane 5 μg of total protein was loaded. Loading was controlled by staining the blots with actin antiserum.