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. 2000 May 15;20(10):3563–3570. doi: 10.1523/JNEUROSCI.20-10-03563.2000

Fig. 7.

Fig. 7.

Mammalian Slowpoke α subunits coimmunoprecipitate with β4 subunits. Shown are Western blots of cell lysates or IPs, using an antibody directed against the V5 epitope that detects V5-His tagged β4 subunits. a, Human proteins. Lane 1, hSlo IP from cells transfected with hSlo and hβ4; lane 2, lysate from same cells as in lane1; lane3, anti-His IP from same cells as in lane 1; lane 4, hSlo IP from cells transfected with hSlo alone; lane 5, hSlo IP from cells transfected with hβ4 alone. b, Mouse proteins.Lane 1, mSlo IP from cells transfected with mSlo and mβ4; lane 2, lysate from same cells as in lane 1; lane3, anti-His IP from same cells as inlane 1.