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. 2000 May 15;20(10):3776–3784. doi: 10.1523/JNEUROSCI.20-10-03776.2000

Fig. 7.

Fig. 7.

CD inhibits FAK and paxillin tyrosine phosphorylation and β1 integrin association. SC/N cocultures were grown for 10 d in CB medium, M medium with and without 0.5 μg/ml CD. Three hundred micrograms of total protein extracts for each culture condition were immunoprecipitated with β1 integrin and phosphotyrosine antibody. Samples were electrophoresed on 10% SDS-PAGE and were immunoblotted with FAK and paxillin antibody.A, Two hundred micrograms of β1 immunoprecipitate from each culture condition was immunoblotted with FAK antibody (left panel). Forty micrograms of β1 immunoprecipitate from each culture condition was immunoblotted with paxillin antibody (right panel). B, Thirty-five micrograms of phosphotyrosine immunoprecipitates from each culture condition was immunoblotted with FAK (left panel) and paxillin (right panel) antibodies. β1 integrin associates with FAK and paxillin in differentiating cocultures but not in CD-treated cocultures. FAK and paxillin tyrosine phosphorylation is lower in CD-treated cocultures than in differentiating cocultures.