Fig. 6.

ADAM8 transcriptional activation by mouse TNF-α in granular cells and NSC19 cells. A, Granular cells (GC) and NSC19 cells were incubated with indicated units of mouse TNF-α for 24 hr; the relative amounts of ADAM8, IRF-1, and L7 (reference) mRNA levels were determined by RT-PCR and visualized by ethidium bromide staining. B, ADAM8 immunofluorescence of NSC19 cells treated with indicated units of TNF-α. Note the staining toward the cell membrane. Even in the presence of 3000 U of TNF-α, NSC19 cells did not show signs of cell damage. Scale bar, 50 μm.