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. 2000 Nov 1;20(21):7964–7971. doi: 10.1523/JNEUROSCI.20-21-07964.2000

Fig. 7.

Fig. 7.

Upregulation of ADAM8 mRNA by exogenous TNF-α. Quantitation of ADAM8 mRNA levels in primary WT and WR astrocytes (A), granular cells (B), and NSC19 cells (C), as determined by RT-PCR.A, Induction of ADAM8 mRNA by TNF-α in primary astrocyte cultures from WT and WR mice. In all cases, values were normalized to those of untreated WT cells, 1.0 by definition.B, ADAM8 induction by TNF-α in granular cells.C, Dose response obtained with recombinant mouse (m) and human (h) TNF-α in NSC19 cells. D, ADAM8 mRNA induction by TNF-α in NSC19 cells after preincubation with cycloheximide (CHX); untreated NSC cells, 1.0 by definition. E, Induction of ADAM8 mRNA by TNF-α in the presence of either IRF-1 antisense (aIRF1) or sense (sIRF1) oligonucleotide. Values were normalized to untreated NSC19 cells with sIRF1. Data were given as mean values of at least three independent experiments. When the same experiment was performed on L929 cells, there was no stimulation of ADAM8 mRNA by TNF-α at the concentrations used above (data not shown).