Fig. 6.

PDBu potentiates evoked release.a, Summary of the destaining protocol used to estimate the proportion of vesicles in the readily releasable pool. Note that PDBu was applied between staining and destaining steps during the second trial. b, Scatter plot comparing release before and after PDBu treatment at individual fluorescent puncta. Values represent the percentage of fluorescence staining released by 30 stimuli at 20 Hz during subsequent trials. c, Data represented as a frequency histogram. d, Frequency histogram showing the enhancement of release by phorbol ester.