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. 2000 Nov 1;20(21):7932–7940. doi: 10.1523/JNEUROSCI.20-21-07932.2000

Fig. 9.

Fig. 9.

Actin cytoskeleton is required for synaptic maintenance of AMPA receptors. A, Actin polymerization is required for synaptic maintenance of AMPA receptors. Cultured rat hippocampal neurons were treated with either DMSO (Lat.A−) or latrunculin A (Lat.A +) followed by cell surface biotinylation (Biotin +, −) as described in Materials and Methods. Samples from cell lysates (Total) and biotinylated samples (PM) were analyzed for GluR1 via anti-GluR1 antibody. Samples of immunoblots (top panel) and a summary of quantitation (bottom panel) are presented. For quantitation, the signals from biotinylated samples were normalized to total GluR1 signals to obtain the ratio of surface expression [PM/Total (%)].DMSO, Lat.A, GluR1 without or with latrunculin A, respectively. n = 4. Error bars indicate SEM.B, Schematic model of AMPA receptor–actin cytoskeleton cross-linking by 4.1N. The interaction of the GluR1 subunit with protein 4.1N and SAP97 may link surface AMPA receptors to the cortical actin cytoskeleton network underneath the synaptic plasma membrane and PSD.