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An example of competitive RT-PCR for 5-HT1A receptor mRNA in the dorsal raphe of 5-HTT mutant mice. A, Autoradiography of DNA sequence gel that resolves RT-PCR products from 5-HT1A mRNA (5-HT1A) and 5-HT1A RNA standard (5-HT1A st). Four concentrations of 5-HT1A RNA standard were used to compete 5-HT1A mRNA (10 ng of total RNA). B, Linear regression curve for calculation of concentration of 5-HT1AmRNA. The intercept of the x-axis represents the concentration of 5-HT1A mRNA.
