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. 2000 Oct 1;20(19):7228–7237. doi: 10.1523/JNEUROSCI.20-19-07228.2000

Fig. 5.

Fig. 5.

PI-3-K is not solely required for the survival-promoting activity of NGF. A, Sympathetic neurons were deprived of NGF in the absence or presence of BAF (50 μm) or were treated with LY294002 (50 μm) in the presence of NGF (50 ng/ml) with and without BAF. The neurons were fixed after 2−4 d. The neurons were stained with toluidine blue, and the number of surviving neurons was counted and graphed as a percentage (compared with age-matched NGF-maintained neurons). Each condition was performed in duplicate in four independent cultures.B, Sympathetic neurons maintained for 5 d in either 50 ng/ml NGF (open symbols) or 10 ng/ml NGF (closed symbols) were deprived of NGF or maintained in NGF in the presence of LY294002 (50 μm). In addition, sympathetic neurons maintained in 10 ng/ml NGF were also deprived of NGF in the presence of BAF (50 μm) to confirm that they could be rescued from apoptosis by a caspase inhibitor. Surviving neurons were counted from duplicate wells of each condition from two independent cultures. Error bars represent SEM.