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. 2000 Oct 1;20(19):7208–7219. doi: 10.1523/JNEUROSCI.20-19-07208.2000

Fig. 6.

Fig. 6.

Delayed Ca2+ deregulation induced in a subpopulation of cells by release of matrix Ca2+ into the cytoplasm occurs in the absence of previous mitochondrial dysfunction. The 7 DIV granule cells were loaded with fura-2 and equilibrated with 50 nmTMRM+. The cells were exposed to 100 μm glutamate/10 μm glycine (G); 2 μg/ml oligomycin (O) and 1 μm FCCP (F) were added where indicated. The traces represent the fura-2 (filled circles trace) and TMRM+ (smooth trace) responses from a single representative soma. A, Cell that regains cytoplasmic Ca2+ homeostasis after FCCP. The FCCP-induced fura-2 spike occurs during release of matrix Ca2+ and its subsequent removal from the cytoplasm. Note the oligomycin-induced fluorescent quenching diagnostic of mitochondrial ATP synthesis before the inhibitor addition.B, Cell that fails to restore a low [Ca2+]c after FCCP. Note that the mitochondria in this cell also generated ATP before the oligomycin addition.