Fig. 5.
RGS6 and RGS11 antagonism of Gβ5γ2 facilitation of Ca currents depends on their GGL domains. RGS6 and RGS11 constructs were cotransfected with Gβ5γ2 at an 8:1 ratio. (RGS6* and RGS11* represent full-length cDNA constructs with mutations in the GGL domain that inhibit binding to β5; see Results and Snow et al., 1999). A, Typical current traces with (trace 2) or without (trace 1) prepulse. B, Summary of facilitation ratios for IBa inhibition after cotransfection with β5γ2 and different RGS constructs. Data are plotted as mean ± SEM; *p < 0.01, one-way ANOVA analysis followed by nonparametric Kolmogorov–Smirnov test (n = 6∼15).