Fig. 6.
RGS protein constructs have different abilities to antagonize IBa inhibition by β5γ2. Various RGS constructs were cotransfected with Gβ5γ2 at an 8:1 ratio. A, Typical current traces with (trace 2) or without (trace 1) prepulse.B, Schematic structures of different RGS protein constructs. Artificial linkers or termini are drawn in thick lines. Hatched boxes, DEP domain; open boxes, GGL domain; filled boxes, hemagglutinin (HA) epitope; gray boxes, RGS domain.C, Summary of facilitation ratios forIBa inhibition after cotransfection with β5γ2 and different RGS protein constructs. Data are plotted as mean ± SEM; *p < 0.01, one-way ANOVA analysis followed by nonparametric Kolmogorov–Smirnov test (n = 8∼15).