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Activity-dependent release of BDNF is regulated by the pattern of stimulation. A, Schematic representation of the stimulation patterns applied to each group of cultures.B, Mean levels of BDNF released from newborn NPG neurons during 60 min of electrical field stimulation with 50 biphasic rectangular pulses of 10 msec, delivered at 5, 10, 20, and 50 Hz, with interburst intervals, respectively, of 0, 10, 15, and 18 sec as shown in A. ***p < 0.001; *p < 0.05; n.s., not significant.
