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. 2000 Oct 1;20(19):7377–7383. doi: 10.1523/JNEUROSCI.20-19-07377.2000

Fig. 4.

Fig. 4.

Epo mimics the lowered O2 effect on dopaminergic differentiation. A, Representative images of TH+ cells grown in the presence or absence of Epo and anti-Epo antibody and in lowered or 20% O2 conditions. All reagents were added to E12 mesencephalic precursor cultures throughout cell expansion and differentiation (10 d total) in lowered or 20% O2. Epo supplementation significantly increased TH+ cell numbers in 20% O2 cultures (n = 6;p < 0.05). Epo neutralizing antibody decreased TH+ cell numbers in both lowered O2 (n = 6;p < 0.01) and 20% O2 cultures (n = 6; p < 0.05). Scale bar, 20 μm. B, Effects of Epo and anti-Epo blocking antibody on dopamine neuron yield. C, Epo positively influences dopaminergic neuron yield in 20% O2 in a dose-dependent manner.