Fig. 1.

Overexpressed truncated FLAG-huntingtin in transfected clonal striatal cells, detected with a FLAG antibody, is localized in the cytoplasm in a meshwork of fine tubules (arrows) and punctate structures (a), in dispersed vacuoles throughout cell bodies (b, c) and neurites (c,arrows), and in vacuoles coalesced in the perinuclear region (d, e, arrow). Full-length FLAG-tagged huntingtin with a normal (f) or expanded (g) repeat also produce FLAG-positive vacuoles (arrows). Vacuolar staining can be obtained using huntingtin antibody Ab585 that recognizes an internal epitope of huntingtin (h,arrow). Huntingtin antibody Ab1 made to the N terminus detects vacuoles in cells expressing untagged