Fig. 4.
Linearization of I–V curves for CabTRP, TNRNFLRFamide, pilocarpine, and CCAP in low external Ca2+. Control I–V curves were obtained in normal external Ca2+ (13 mm). Low-calcium saline contained 0.1 mmCa2+. Curves for CabTRP, TNRNFLRFamide and pilocarpine were obtained from SEVC recordings of cultured neurons. The CCAP curve was obtained from a TEVC recording of the LP neuron (semi-intact preparation) in the presence of 10 μm PTX and 0.1 μm TTX. In each individual graph, control and low-Ca2+ responses are from the same neuron. Each graph, however, shows results from a separate neuron. CabTRP (10−6m), TNRNFLRFamide (10−6m), and pilocarpine (3 × 10−5m) were bath-applied. CCAP (10−4m) was pressure-applied to the ganglion neuropil.