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Expression of ACT mRNA in human astrocytes. Human astrocytes were treated with IL-1 (5 ng/ml), TNFα (10 ng/ml), or LPS (1 μg/ml). RNA was isolated after 18 hr and subjected to Northern blot analysis using ACT cDNA as a probe. Bottom panelshows 18S RNA stained with ethidium bromide on the membrane.
