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. 2000 Oct 15;20(20):7510–7516. doi: 10.1523/JNEUROSCI.20-20-07510.2000

Fig. 3.

Fig. 3.

Detailed analysis of the putative IL-1/TNF response element of the ACT gene. Human astrocytes were transfected with p'a'StCAT, pΔBglIIACTCAT, pΔHindIIIACTCAT, pΔSphIACTCAT, pSSCAT, prSSCAT, p1EECAT, or p2EECAT (A); p1EECAT, pER-SCAT, pdelee1EECAT, pee1CAT, pee2CAT, pΔACTCAT, pΔ5ACTCAT, or pΔ3ACTCAT (B). An internal control plasmid pCH110 encoding β-galactosidase was included in all transfection experiments. Cells were treated with cytokines and harvested as described in the legend to Figure 2. OPmarks 244-bp-long ACT promoter. Restriction sites forBglII (B), EcoO109I (O), EcoRI (R), EcoRV (E), HindIII (H), and SacI (S) are indicated. Nucleotide sequence of the ACT IL-1/TNF response element is shown (C). The NF-kB and AP-1 elements are boxed.