Skip to main content
. 2000 Oct 15;20(20):7510–7516. doi: 10.1523/JNEUROSCI.20-20-07510.2000

Fig. 4.

Fig. 4.

The untranslated region of ACT mRNA does not confer any response to IL-1 and TNF. Human astrocytes were transfected with either ptkCATΔEH, pACT-244CAT, p-2431ACT(ex-in-ex)CAT, p-244un1CAT, or p-244un2CAT and an internal control plasmid pCH110 encoding β-galactosidase. Cells were treated with cytokines and harvested as described in the legend to Figure 2. CAT activities were normalized to β-galactosidase activities (cpm/unit × 10−3). The two variants of the untranslated region (un1, un2), first intron (int), and first and second exon (exI,II) of the ACT gene are marked. tkindicates thymidine kinase minimal promoter from ptkCATDEH.