Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
EMSA of nuclear extracts from human astrocytes using fragments from the IL-1/TNF response element. Human astrocytes were incubated with IL-1 or TNF for 40 min (A) or 4 hr (B). Nuclear extracts were prepared and analyzed using 32P-labeled double-stranded fragments derived from the IL-1/TNF response element;5′NF-kB, 3′NF-kB, andAP-1. Gels were exposed for 48 (A) or 24 (B) hr. Asterisks indicate IL-1-, TNF-induced bands.
