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The IL-1-, TNF-induced protein is recognized by anti-NF-kB antibodies. Human astrocytes were stimulated with IL-1 or TNF for 40 min. Nuclear extracts were prepared and analyzed using a 5′NF-kB double-stranded oligonucleotide. Extracts were incubated with anti-NF-kB antibodies or normal rabbit serum (NRS) for 10 min when indicated. Arrow indicates position of the IL-1-, TNF-induced band. Asterisk marks super-shifted complexes.
