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Expression of IkB inhibits activation mediated by the ACT IL-1/TNF response element. Human astrocytes were transfected with pΔ5ACTCATH, IkB expression vector, and β-galactosidase expression vector as an internal control for transfection efficiency. One day after transfection, cells were stimulated with the indicated cytokines, cultured for another 24 hr, and harvested. CAT activities were normalized to β-galactosidase activities (cpm/unit × 10−3).
