Fig. 1.
Characterization of G-protein antibodies.A, All antibodies gave an appropriate ∼40 kDa band for chick brain protein (10 μg/lane). Lane 1, Monoclonal anti-Go (Ab-1); 2, monoclonal anti-Go (Ab-2); 3, polyclonal anti-Go (J. K. Northup); 4, polyclonal anti-Gi3 (Calbiochem); 5, polyclonal anti-Gi1–3 (Santa Cruz Biotechnology); 6, polyclonal anti-Go+i3 (DuPont); 7, polyclonal anti-Gq/11 (DuPont); 8, polyclonal anti-Gq/11 (Santa Cruz Biotechnology); 9, polyclonal anti-Gq/11 (Calbiochem); 10, polyclonal anti-G12,13 (Gutkind); 11, polyclonal anti-Gs (DuPont); 12, polyclonal anti-Gz (Calbiochem). B, Blot of antibodies against the recombinant Gα subunits: Go, Gs, Gq, G11, Gi1, Gi2, Gi3, and G13, as well as a G-protein mixture that includes Gz.