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Localization of PTX-insensitive G-proteins: Gs. Gαs predominantly localized to the calyx cytoplasm. Calyx terminals are identified by SV2 (red) in each panel pair. A, Attached calyces were as stained as the postsynaptic neuron with reduced staining at the interface. B, Isolated calyces showed bright GαS staining along the axon that decreased at the terminal region.
