Fig. 9.

Localization of PTX-insensitive G-proteins: G_z. Attached (A) and isolated (B) calyces had bright fibrous staining for G_z that coursed through the axon and partially into the terminal and was inversely correlated with SV2 (red, left panel). This staining pattern was suggestive of colocalization with the cytoskeleton. C, Tubulin α (red, left panel) stained predominantly near the calyx membrane and, hence, did not colocalize with the predominantly intracellular staining of G_z (yellow; right panel). D, G_z showed almost perfect colocalization (yellow, right panel) with the phosphorylated 200 kDa subunit of neurofilament protein (red, left panel).