Table 1.
Antibodies used in this study
| Antibody | Source | Immunostain dilution | Western blot dilution |
|---|---|---|---|
| Poly anti-Gαo/i | J. K. Northup | 1:200 | 1:1000 |
| Poly anti-Gαil−3 | Santa Cruz (sc262) | 1:100 | 1:1000 |
| Poly anti-Gαo+i3 | DuPont (NEI-803) | 1:100 | 1:1000 |
| Poly anti-Gαi3 | Calbiochem (371729) | 1:100 | 1:1000 |
| Mono anti-Gαo (Ab-1) | Lab Vision | 1:200 | 1:1000 |
| Mono anti-Gαo (Ab-2) | Lab Vision | 1:200 | 1:1000 |
| Mono anti-Gαi1 | Lab Vision | 1:100 | 1:1000 |
| Mono anti-Gαi2 | Lab Vision | 1:100 | 1:1000 |
| Poly anti-Gαq/11 | DuPont | 1:100 | 1:1000 |
| Poly anti-Gαq/11 | Calbiochem | 1:100 | 1:1000 |
| Poly anti-Gαq/11 | Santa Cruz | 1:100 | 1:1000 |
| Poly anti-Gα12,13 | Gutkind | 1:100 | 1:1000 |
| Poly anti-Gαs | DuPont | 1:100 | 1:1000 |
| Poly anti-Gαz | Calbiochem | 1:100 | 1:500 |
| Mono SV2 | DSHB | 1:0–1:1 | |
| Poly SV2A | StressGen | 1:200 | |
| Mono neurofilament | DSHB | 1:1 | |
| Mono tubulin α | Lab Vision | 1:100 | |
| Mono tubulin β | Lab Vision | 1:100 |