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Confocal fluorescence micrographs of cultured rat sympathetic neurons treated for 5 d with BMP7 immunostained with antibodies specific for the −IRE (A, C) and +IRE (B, D) species of DMT1. Peptides to the C-terminal sequence, used to generate the antibodies, for either the −IRE (C) or +IRE (D) forms of DMT1 were added before immunostaining. In both cases, addition of peptides attenuated the intensity of the immunostaining of the neurons.
