Fig. 8.

Dominant-negative Akt induced caspase activation in primary cultures of A2B5-positive oligodendrocyte progenitors. Oligodendrocyte progenitors were mock-infected or infected with adenovirus expressing wt-Akt (50 MOI) or AA-Akt (10 MOI) for 24 hr as described in Materials and Methods. After infection, cells were subjected to caspase activation analysis (green) and A2B5 staining (red). Condensed nuclei were detected by DAPI staining (indicated by arrows). Representative data from one of three independent experiments is shown.