Fig. 2.

Neurofilament whole-mount immunohistochemistry of the skin from the head of developing embryos shows that axons of the ophthalmic branch of the TG are less extensive and complex in the p75 −/− embryos. Shown is a sagittal view of the head in the controls (A, C, E,G) and p75 mutants (B, D,F, H) at ages E11.5 (A, B), E12.5 (C,D), E13.5 (E, F), and E14.5 (G, H). The contralateral side of the head and the brain have been removed inE–H to increase transparency of the skin. Asterisks indicate location of the eye. Arrows point to axons of the ophthalmic branch. Photographs of whole mounts were taken using a dissecting microscope at 40× magnification.