Fig. 1.
Expression of 7D5 in the brain. A, Expression profile of 7D5 during cerebellar development. Poly(A+) RNAs from P0 and P21 mouse cerebellum were electrophoresed (2 μg/lane), transferred onto a nylon membrane, and hybridized with a 32P-labeled cDNA (7D5). The expression of 7D5 was characterized by downregulation in postnatal development. G3PDH was used as a control for loading of RNA. B, Tissue distribution of 7D5 in adult mice. An adult mouse multiple tissue Northern blot (Clontech) was hybridized with the probe mentioned above. 7D5 was specifically expressed in the brain. β-actin was used as a control for loading of RNA. Arrowheads inA and B indicate 7D5 signals.