Fig. 4.

Forskolin stimulates CREB phosphorylation in the amygdala. Forskolin (50 μm) or vehicle (0.1% DMSO) was applied 90 min after slice dissection and perfused for 15 min; the slices were fixed 60 min after forskolin application. An increase in phospho-CREB immunoreactivity in the lateral amygdala was obtained in forskolin-treated slices (n = 8; bottom panels) as compared with the vehicle-treated slices (n = 8; top panels).Left, Lower magnification. Scale bar, 500 μm.Right, Higher magnification. Scale bar, 50 μm.