Fig. 5.

Overexpression of DCAMKL1 in cultured neural cells leads to a microtubule bundling phenotype that is resistant to depolymerization with either cold or colchicine treatment.A–C, Transfection of neural cells (in this case a likely glial cell based on morphology) with lacZ-encoding vector leads to no change in microtubule bundling and a diffuse distribution of lacZ within the cytoplasm. D–F, Transfection of cells with DCAMKL1-Myc leads to microtubule bundling (F, arrows). Scale bar, 10 μm. G–I,Overexpression of DCAMKL1 stabilizes microtubules to colchicine treatment (H, I, arrows), whereas surrounding untransfected cells (data not shown) or cells expressing low levels of DCAMKL1 have destabilized microtubules. J–L,Microtubule bundles induced by overexpression of DCAMKL1 are partially resistant to cold (0°C) treatment (K, L, arrows), whereas microtubules in neighboring cells are disrupted (data not shown). Scale bars: A, D, G, J, 10 μm.