Fig. 7.

Triple labeling confirms that BrdU-positive cells mature into neurons. Rats received BrdU injections 4 d after the last ECS treatment and were killed 4 weeks later. A representative confocal laser-scanning image (66×) of a section from a fluoxetine-treated rat that has been triple-labeled with BrdU (A, green; BrdU-positive cells indicated byarrows), GFAP (B, blue), and NeuN (C, red). The merged image (D) demonstrates cells that are double-labeled in the GCL for BrdU and NeuN but not GFAP.