Fig. 6.

Low levels of agrin expression affect minis and evoked responses in hippocampal neurons. A, Representative examples of excitatory currents evoked by action potentials in neurons treated with agrin sense (control) (left) or antisense (right) oligonucleotides (Vm = −60 mV). B, Miniature events recorded from control (left) and antisense oligonucleotide-treated (right) neurons in the presence of TTX. Sweep numbers are shown on the right of the corresponding traces. In the antisensetraces, only two minis were detected between sweeps 354 and 378. C, Quantification of the effects of the oligonucleotide treatments on the frequency of minis [left; data from n = 12, 7, and 10 experiments for control (C), sense (S), and antisense (AS) conditions, respectively] and the amplitude of the evoked EPSCs (right; data from n = 12, 8, and 6 experiments for control, sense, and antisense conditions, respectively) (mean ± SEM).