Fig. 4.

Colocalization of Kv4.2 and filamin in cultured hippocampal neurons. Cultured hippocampal neurons were double-immunolabeled by using anti-Kv4.2 (A, C, E), anti-synaptophysin (B, D), and anti-filamin (F) antibodies. A–D, Double immunolabeling of endogenous Kv4.2 and synaptophysin shows that Kv4.2 is distributed in a punctate pattern along dendrites, matching closely with that of synaptophysin. E, F, Double immunolabeling of endogenous Kv4.2 and filamin shows that Kv4.2 and filamin colocalize in a punctate pattern along the dendrites. Large insets in E and F represent high magnification view of small insets in eachpanel, respectively.