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. 2000 Dec 1;20(23):8727–8735. doi: 10.1523/JNEUROSCI.20-23-08727.2000

Fig. 8.

Fig. 8.

Distribution and differentiation of control transplants at 6 weeks after transplantation in the adult rat hippocampus. Immunofluorescence for BrdU (green) is combined with labeling for NeuN (red) and GFAP (blue). A, Freeze-thawed, BrdU-labeled, clonally expanded adult spinal cord stem-like cells show minimal dispersion from the injection tract (indicated byarrowheads). Intense GFAP staining is observed surrounding the transplant. The boxed area is shown at higher magnification in B. B,Freeze-thawed, BrdU-labeled nuclei in the GCL do not express NeuN (arrowhead) or GFAP (arrows).C, BrdU-labeled fibroblast transplant into the hippocampus is shown. Minimal dispersion of the transplanted fibroblasts from the injection tract is seen. Arrowheadsindicate the needle tract. D, BrdU-labeled cells in the GCL (arrowhead) do not colocalize with NeuN or with GFAP (arrows). Scale bars: A, C, 100 μm;B, D, 25 μm.