Skip to main content
. 2000 Dec 1;20(23):8762–8770. doi: 10.1523/JNEUROSCI.20-23-08762.2000

Fig. 4.

Fig. 4.

Confocal immunofluorescence analysis of neuregulin, MuSK, and agrin at adult rat gastrocnemius NMJ. En face (A1, D1, G1,J1) and side view (B1, E1,H1, K1) images of NMJs stained with Bodipy-αBtx. The NMJs were double-labeled with antibodies against neuregulin-1914 (A2, B2), neuregulin-HM 24 (D2, E2), MuSK (G2,H2), and agrin (J2, K2) and visualized using a Cy3-conjugated secondary antibody. Color overlays are shown with α-Btx in green and the respective antibody in red(A3–K3). Schematics of these distributions are shown in C, F,I, and L. The neuregulin 1914 antibody recognizes neuregulin isoform(s) localized to the axon terminal (arrow) and the secondary folds (arrowhead). The neuregulin HM 24 antiserum recognizes neuregulin isoform(s) localized to the Schwann cell. MuSK is enriched in the primary gutter. Agrin appears enriched in the axon terminal and the basal lamina. Scale bar, 10 μm.