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Complement depletion with cobra venom factor treatment at a dose of 500 μg/kg intraperitoneally at day 0 and thereafter at a dose of 250 μg/kg on days 2, 4, 6, 8, 10, and 12. Complement hemolytic activity was measured through lysis of sheep erythrocytes sensitized with IgM antibody and represented relative to a control value of 100% (mean ± SEM).
