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. 2000 May 1;20(9):3354–3368. doi: 10.1523/JNEUROSCI.20-09-03354.2000

Fig. 2.

Fig. 2.

EGFP expression pattern in the hippocampus of GIN mice. a, Montage exemplifying the EGFP expression pattern in the adult dorsal hippocampus, created from overlapping photomicrographs of a 50-μm-thick section from an homozygotic mouse.Asterisk denotes the plexus of EGFP-expressing axonal terminals in SLM of area CA3. b–d, Higher magnification images of the areas denoted by the respective letters in a. b, Cells in SO of area CA3.c, Cells from SO of area CA1, one of which shows classical O-LM cell-type morphology (arrow).d, Cell from SR of area CA1 with long tapering dendrites. e, EGFP-expressing cells in the hilus of the dentate gyrus near the hilar-CA3 border. f, g, EGFP expression in area CA3 of the adult hippocampus from a section immunohistochemically processed for the neuron-specific nuclear protein NeuN. f, Visualization of EGFP expression alone.g, Simultaneous visualization of EGFP and NeuN expression using a double filter set. All EGFP-expressing cells coexpressed NeuN, as indicated by the bright yellownuclei at the centers of their somata. It should be noted that the NeuN signal shows some bleedthrough when viewing with the GFP filter set (compare SP from a with f). Strata abbreviations: A, alveus; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; SLM, stratum lacunosum-moleculare;SM, stratum moleculare; SG, stratum granulosum; H, hilus of the dentate gyrus;SL, stratum lucidum. Scale bars: a, 200 μm; e, 50 μm (used forb–e); g, 100 μm (used for f and g).