Fig. 3.

Methyl group incorporation into brain DNAin vivo in Dnmt^+/+(A) and Dnmt^S/+mice (B) after 30 min middle cerebral artery occlusion. On reperfusion,l-[methyl-³H]methionine was administered and [³H]methyl group incorporation was measured after 12 hr. DNA methylation was significantly increased in ischemic cortex (COR) and striatum (STR) compared with the contralateral side inDnmt^+/+ (A) but not Dnmt^S/+ mice (B). Levels in cerebellum (CER) are shown for comparison. n = 4 and 5 animals. Mean ± SE. *p < 0.05. Paired Student'st test.