Fig. 5.

Effect of the interference with Raf in the number and distribution of the Islet-1/2-positive neurons. Retinas infected at E4.5 with the indicated viral constructs were processed 72 hr after injection for Islet-1/2 immunostaining in either dissociated whole retina cells (A) or cryosections (B). In A, the results correspond to the mean ± SD value of the percentage of labeled cells (500 total cells were counted in duplicate for each of 3 infected embryos per viral construct). In B, the results shown are the percentages of labeled cells in the different retinal layers (4 sections of 3 infected embryos per viral stock were counted, and the scores of fields such as that presented in Fig.4I were added). GCL, Ganglion cell layer; INL, prospective inner nuclear layer.