Fig. 5.

A–H, LarvalVoila¹ expression pattern shown with lacZ (A–E, H) and GFP reporters (F, G).A–C, Second larval instar. Expression is seen in the antennomaxillary complex (A,amc) and in an anterior and posterior group of pharyngeal sensilla (A, ap,pp). In the AMC, dendritic staining extends to the gustatory terminal organ (B, to) but not the olfactory dorsal organ (B, do). Intense labeling occurs in the mushroom bodies (C,arrowheads; β/γ lobes, arrow) and in additional scattered cells in the brain and ventral nerve cord.D–H, Third larval instar. Peripheral expression is similar as in second instar (D). Neurons are labeled exclusively in the terminal organ (E, F), including the so-called dorsolateral group of sensilla (E,arrowhead), but not in the dorsal organ. Massive staining occurs in the mushroom bodies (G,arrowheads; β/γ lobes, arrow) and in many cells of the brain and thoracic ganglia (H,arrowheads). The neuropil in G was counterstained by mAb nc82. Scale bar, 50 μm.