Fig. 8.

Number of IκBα-, TNF-α-, and CD14-positive cells in the caudate putamen of animals pretreated or not with an anti-rTNF-α-neutralizing antibody before the systemic challenge with the endotoxin LPS. Rats received an intracerebroventricular injection of vehicle (Veh; 5 μl for 2 min), anti-rTNF-α-neutralizing antibody (Ab; 2 μg/5 μl for 2 min), or goat serum as a control IgG (5 μl/2 min) 10 hr before an intraperitoneal injection of either a vehicle solution or LPS (1.5 mg/kg, b.w.). Positive cells were counted in three different areas (1.0 mm²) of the caudoputamen under dark-field illumination at a magnification of 10×. Data are reported as mean values of cells/1.0 mm² (± SEM) for experimental and control animals killed 6 hr after the intraperitoneal injection of the endotoxin or its vehicle solution. *Significantly different (p < 0.05) from their corresponding vehicle-treated groups.